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- Genetically modified cell lines best reflect MOA (Mechanism of Action)
- Higher activity and larger assay window for robust and reproducible cell-based bioassay
- Comprehensive application data to support assay development and validation
- Full tracible record, stringent quality control and validated cell passage stability
- Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
- Global commercial license assistance whenever regulatory filing is required
描述（Description）
The Human THRB (Luc) HEK293 Reporter Cell was engineered to not only express thyroid hormone response element (TRE), but also express the receptor full length human THRB (Gene ID: 7068), which can drive luciferase expressing systems by thyroid hormone (TH) or thyroid hormone receptor (THR) agonists stimulation. In the absence of TH or THR agonists, the THRB receptor is not activated and luminescence signal is low. In the presence of TH or THR agonists, the THRB pathway-activated luminescence can be detected in a dose-dependent manner.
应用说明（Application）
• Screen for THR-β-selective agonists.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Complete Growth Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
质量管理控制体系（QMS）
数据展示
Application
Bioactivity analysis of THR-β-selective agonist (RLU).
The Human THRB (Luc) HEK293 Reporter Cell and Human THRA (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF180) were incubated with serial dilutions of Resmetirom (a THR-β-selective agonist), respectively. The EC50 of Resmetirom determined on Human THRB (Luc) HEK293 Reporter Cell was approximately 5.5 μM.Protocol
Bioactivity analysis of THR-β-selective agonist (FOLD).
The Human THRB (Luc) HEK293 Reporter Cell and Human THRA (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF180) were incubated with serial dilutions of Resmetirom (a THR-β-selective agonist), respectively. The max induction fold of Resmetirom determined on Human THRB (Luc) HEK293 Reporter Cell was approximately 31.96, and on Human THRA (Luc) HEK293 Reporter Cell was approximately 1.92, which exhibited higher potency and selectivity for THR-β over THR-α.Protocol
Signaling Bioassay
Response to thyroid hormone (RLU).
This reporter cell was incubated with serial dilutions of Liothyronine (a dual THR-α and THR-β agonist). The EC50 of Liothyronine was approximately 0.0042 μM.Protocol
Response to thyroid hormone (FOLD).
This reporter cell was incubated with serial dilutions of Liothyronine (a dual THR-α and THR-β agonist). The max induction fold was approximately 90.99.Protocol
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背景介绍
重要声明
Limited Use&License Disclosure
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.
- If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
- Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
- This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use. You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
- ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
- ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
- Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order.cn@acrobiosystems.com for further details.
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