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产品概述（Product Details）
ClinMax™ Human IL-12/IL-23 p40 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-12/IL-23 p40 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human IL-12/IL-23 p40 in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
Assay TypeSandwich-ELISAAnalyteIL-12/IL-23 p40Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity<15.625 pg/mLAssay Time2hrSample volume50 μLRange31.25 pg/mL-2000 pg/mlSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code95/544Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCEA203-C01Pre-coated Anti- IL-12/IL-23 p40 Antibody Microplate1 plateCEA203-C02Human IL-12/IL-23 p40 Standard20μg ×2CEA203-C03Biotin-Anti-IL-12/IL-23 p40 Antibody Con. Solution100 μLCEA203-C04Biotin-Antibody Dilution Buffer8 mLCEA203-C05Streptavidin-HRP Con. Solution500 uLCEA203-C06Streptavidin-HRP Dilution Buffer15 mLCEA203-C0720× Washing Buffer50 mLCEA203-C08Sample Dilution Buffer15 mL ×2CEA203-C09Substrate Solution12 mLCEA203-C10Stop Solution6 mL质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-12/IL-23 p40 is less than 15.625 pg/mL.
批内差异（Intra-Assay Statistics）
Ten replicates of each of 4 samples containing different IL-12/IL-23 p40 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
Five samples containing different concentrations of IL-12/IL-23 p40 were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant IL-12/IL-23 p40 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-12/IL-23 p40 for serum samples is 103.2%.
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