TNF-alpha [Biotinylated] : TNFR2 Inhibitor Screening ELISA Kit

产品详情

ID	Components	Size
EP147-C01	High-bind Plate	1 plate
EP147-C02	Human TNFR2	15 μg
EP147-C03	Biotinylated Human TNF-alpha	10 μg
EP147-C04	Anti-TNF-alpha Neutralizing Antibody	20 μg
EP147-C05	Streptavidin-HRP	10 μg
EP147-C06	Coating Buffer	12 mL
EP147-C07	10xWashing Buffer	50 mL
EP147-C08	Blocking Buffer	50 mL
EP147-C09	Substrate Solution	12 mL
EP147-C10	Stop Solution	7 mL

产品概述（Product Overview）
TNF-alpha [Biotinylated] : TNFR2 Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human TNF-alpha binding to human TNFR2. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human TNF-alpha binding to human TNFR2.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
质量管理控制体系（QMS）

典型数据-Typical Data
Please refer to DS document for the assay protocol.
INHIBITION OF TNF-alpha [Biotinylated]: TNFR2 BINDING BY ANTI-TNF-alpha NEUTRALIZING ANTIBODY
Serial dilutions of Anti- TNF-alpha Neutralizing antibody (Catalog # EP147-C04) (1:1 serial dilution, from 10 μg/mL to 0.02μg/mL) was added into Biotinylated TNF-alpha: TNFR2 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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