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分子别名（Synonym）
CD7,GP40,TP41,LEU-9,Tp40
表达区间及表达系统（Source）
Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (CD7-HA2H9) is produced via conjugation of AF488 to Human CD7 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD7 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
Predicted N-terminus: Ala 26
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.3 kDa.
偶联（Conjugate）
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
活性（Bioactivity）-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) at 1 μg/mL (100 μL/well) can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.05-2 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
活性（Bioactivity）-FACS
1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD7 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
稳定性（Stability）
Alexa Fluor 488-Labeled Human CD7 Protein, His Tag (Cat. No. CD7-HA2H9) is stable at 25℃ for 48 hours, equivalent to store at -70℃ for 2 years and freezing and thawing 3 times without performance reduction.
Binding activity of three different lots of Alexa Fluor 488-Labeled Human CD7 Protein, His Tag against Anti-CD7 CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
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