产品详情
分子别名(Synonym)
HLA-C*07:02:01 & B2M & RYR
表达区间及表达系统(Source)
Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (HLR-H52W2) is expressed from human 293 cells (HEK293). It contains AA Cys 25 - Ile 308 (HLA-C*07:02:01) & Ile 21 - Met 119 (B2M) & RYRPGTVAL peptide (Accession # P10321 (HLA-C*07:02:01) & P61769 (B2M) & RYRPGTVAL).
Predicted N-terminus: Cys 25 & Arg
Request for sequence蛋白结构(Molecular Characterization)
Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein is produced by co-expression of HLA and B2M loaded with RYR peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.1 kDa and 13.8 kDa. The protein migrates as 42-45 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Biotin
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
活性(Bioactivity)-SPR
Biotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer) (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL3, Fc Tag (Cat. No. KI3-H5258) with an affinity constant of 2.40 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
ProtocolBiotinylated Human HLA-C*07:02:01&B2M&RYR (RYRPGTVAL) Complex Protein (Monomer) (Cat. No. HLR-H52W2) immobilized on SA Chip can bind Human KIR2DL2, Fc Tag (Cat. No. KI2-H5255) with an affinity constant of 1.01 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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