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产品参数（Product Specifications）
Assay TypeSandwich-ELISAAnalyteAAV9Format96TReactivity/Regulatory StatusRUOSensitivity≤100ng/mLStandard Curve Range0.313 ng/mL-20 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the quantitative determination of anti-AAV9 antibodies in serum.Sample volume100 uL产品概述（Product Overview）
Anti-AAV8 Antibody ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the Anti-AAV8 Antibody in serum. The kit is designed to provide reliable solutions for the immunogenicity studies and enrollment screening of AAV gene therapy products.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizePAV009-C01Pre-coated AAV9 Capsid Protein Microplate1 platePAV009-C02Anti-AAV9 Antibody Standard2.5 μgPAV009-C03Biotin-AAV9 Capsid Protein10 μgPAV009-C04Streptavidin-HRP10 μgPAV009-C0510xWashing Buffer50 mLPAV009-C062xDilution Buffer50 mLPAV009-C07Substrate Solution12 mLPAV009-C08Stop Solution7 mL质量管理控制体系（QMS）
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
Detection of anti-AAV9 antibodies by sandwich-ELISA Assay.
Immobilized AAV9 Capsid Protein can bind Anti-AAV9 Antibody in 1:50 human serum, and then add Biotin-AAV9 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 15.6 ng/mL.
The specific binding of PAV-A009 to Anti-AAV3/5/8 antibody was detected. The results showed that PAV-A009 only binds specifically to Anti-AAV9 antibody.
批内差异（Intra-Assay Statistics）
Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
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