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分子别名（Synonym）
Disintegrin and metalloproteinase domain-containing protein 9 (EC:3.4.24.-) Cellular disintegrin-related protein,Meltrin-gamma,Metalloprotease,disintegrin,cysteine-rich protein 9,Myeloma cell metalloproteinase,ADAM9,KIAA0021, MCMP, MDC9, MLTNG
表达区间及表达系统（Source）
Human ADAM9, His Tag (AD9-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 206 - Asp 697 (Accession # Q13443-1).
Predicted N-terminus: Ala 206
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.2 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human ADAM9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human ADAM9, His Tag (Cat. No. AD9-H52H7) at 1 μg/mL (100 μL/well) can bind Izeltabart with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Izeltabart at 1 μg/mL (100 μL/well) can bind Human ADAM9, His Tag (Cat. No. AD9-H52H7) with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Anti-ADAM9 Antibody captured on Protein A Chip can bind Human ADAM9, His Tag (Cat. No. AD9-H52H7) with an affinity constant of 0.786 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >10 pmol/min/µg (QC tested).
Protocol
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