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Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

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CDG-H5253-100ug
¥3675.00
CDG-H5253-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD3 epsilon & CD3 gamma,CD3E & CD3G

  • 表达区间及表达系统(Source)

    Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)).

    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD3E & CD3G Structure

    Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 38.3 kDa (CD3E) and 36.9 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 43-55 kDa under reducing (R) condition, and 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    CD3E & CD3G SDS-PAGE

    Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.

  • SEC-MALS

    CD3E & CD3G SEC-MALS

    The purity of Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) is more than 90% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CD3E & CD3G ELISA

    Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.8-3 ng/mL (QC tested).

    Protocol
  •  CD3E & CD3G ELISA

    Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti Human CD3 with a linear range of 0.8-2 ng/mL (Routinely tested).

    Protocol
  •  CD3E & CD3G ELISA

    Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.8-3 ng/mL (Routinely tested).

    Protocol
  •  CD3E & CD3G ELISA

    Immobilized Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-H5253) at 1 μg/mL (100 μL/well) can bind Bispecific BCMA×CD3 T cell-engaging Antibody with a linear range of 0.8-3 ng/mL (Routinely tested).

    Protocol

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背景介绍

T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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