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分子别名（Synonym）
TNFRSF25,DR3,APO3,DDR3,TNFRSF12,WSL,WSL1
表达区间及表达系统（Source）
Biotinylated Human DR3 Protein, Fc,Avitag (DR3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 47.2 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Biotin
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human DR3 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.005-0.625 μg/mL (QC tested).
Protocol
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Protocol
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 1.976 μg/mL (Routinely tested).
Protocol
Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 3.06 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) captured on Biotin CAP-Series S Sensor Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 1.02 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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