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分子别名（Synonym）
IFN-alpha 2b,IFNA2B,IFN-alpha 2 (alpha 2b),interferon alpha 2b
表达区间及表达系统（Source）
Human IFN-alpha 2b, Fc Tag (IFB-H5253) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 188 (Accession # P01563-1).
Predicted N-terminus: Cys 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.7 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
95% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
产品展示
电泳（SDS-PAGE）
Human IFN-alpha 2b, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IFN-alpha 2b, Fc Tag (Cat. No. IFB-H5253) is more than 90% and the molecular weight of this protein is around 95-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IFNAR2, His Tag (Cat. No. IF2-H5224) at 5 μg/mL (100 μL/well) can bind Human IFN-alpha 2b (K46R), Fc Tag (Cat. No. IFB-H5253) with a linear range of 2.4-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human IFN-alpha 2b (K46R), Fc Tag (Cat. No. IFB-H5253) immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).
Protocol
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