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分子别名（Synonym）
IL-11,Interleukin-11,AGIF,Oprelvekin,IL11
表达区间及表达系统（Source）
Human IL-11 Protein, His Tag (IL1-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20809-1).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 21.0 kDa. The protein migrates as 23-29 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
Negative
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Citrate buffer, pH3.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human IL-11 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL-11 Protein, His Tag (Cat. No. IL1-H5243) at 5 μg/mL (100 μL/well) can bind Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) with a linear range of 1-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human IL-11 Protein, His Tag (Cat. No. IL1-H5243) on HIS1K Biosensor, can bind Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) with an affinity constant of 0.177 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-CELL BASE
Human IL-11 Protein, His Tag (Cat. No. IL1-H5243) stimulates proliferation of STAT3 (Luc) HEK293 Reporter Cell. The specific activity of Human IL-11 Protein, His Tag is＞8.00 x 10^3 U/mg (QC tested).
Protocol
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