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FITC-Labeled Human IL-13 R alpha 2 Protein, His Tag

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IL2-HF2H3-25ug
¥3350.00
IL2-HF2H3-200ug
¥20790.00
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产品详情

  • 分子别名(Synonym)

    IL13RA2,CD213A2,CT19,IL-13R,IL13BP

  • 表达区间及表达系统(Source)

    FITC-Labeled Human IL-13 R alpha 2, His Tag (IL2-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). It is the FITC labeled form of Human IL-13 R alpha 2, His Tag (IL2-H52H5).

    Predicted N-terminus: Asp 27

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    IL-13 R alpha 2 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.0 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    IL-13 R alpha 2 SDS-PAGE

    FITC-Labeled Human IL-13 R alpha 2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     IL-13 R alpha 2 ELISA

    Immobilized Human IL-13, His Tag (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-13 R alpha 2, His Tag (Cat. No. IL2-HF2H3) with a linear range of 0.313-1.25 μg/mL (QC tested).

    Protocol

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背景介绍

Interleukin-13 receptor subunit alpha-2 is also known as IL13Rα2, IL13Ra2 cluster of differentiation 213A2, CD213A2, CT19, IL-13R, IL13BP, and is a membrane bound protein that in humans is encoded by the IL13RA2 gene. IL13Rα2 is closely related to IL13Rα1, a subunit of the interleukin-13 receptor complex. This protein binds IL13 with high affinity, but lacks any significant cytoplasmic domain, and does not appear to function as a signal mediator. It is, however able to regulate the effects of both IL13 and IL4, despite the fact it is unable to bind directly to the latter. It is also reported to play a role in the internalization of IL13. IL13Rα2 is a component of the cell surface receptors, however, the majority exists in intracellular pools and in soluble form, and thus plays an opposite role as a potent IL13 antagonist compared with IL13Rα1. It also functions as an inhibitor of IL4-dependent pathway probably through the physical interaction between the short intracellular domain of and cytoplasmic domain of IL13Rα2 and the IL4Rα chain. In spite of the failed STAT signaling function, IL13Rα2 dose induce TGF-beta production and fibrosis. Additionally, IL13Rα2has been reported to be abundantly and specifically overexpressed in glioblastoma multiforme.

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-13 receptor subunit alpha-2

  • 中文全称:

    白细胞介素-13受体α2亚基

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    7 详情

  • 最高研发阶段:

    临床一期

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