产品详情
分子别名(Synonym)
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表达区间及表达系统(Source)
Rhesus macaque PCSK9, His Tag (PC9-C52H2) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # A8T666).
Predicted N-terminus: Gln 31
Request for sequence蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.4 kDa respectively. The protein migrates as 17 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
Unconjugated
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
Rhesus macaque PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%undefined.
活性(Bioactivity)-ELISA
Immobilized Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) at 1 μg/mL (100 μL/well) on Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) precoated (0.1 μg/well) plate can bind Evolocumab with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
活性(Bioactivity)-BLI
Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Rhesus macaque PCSK9, His Tag (Cat. No. PC9-C52H2) with an affinity constant of 1.36 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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