产品详情
分子别名(Synonym)
Prefusion glycoprotein F0/pre-F protein (RSV)
表达区间及表达系统(Source)
FITC-Labeled Human respiratory syncytial virus A (strain A2) Pre-F0, His Tag (RSF-V52H3) is expressed from human 293 cells (HEK293).
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 56.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联(Conjugate)
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记(Labeling)
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
数据展示
电泳(SDS-PAGE)
FITC-Labeled Human respiratory syncytial virus A (strain A2) Pre-F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA
Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human respiratory syncytial virus A (strain A2) Pre-F0, His Tag (Cat. No. RSF-V52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
用户评价 发表评论
