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分子别名（Synonym）
SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
表达区间及表达系统（Source）
PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) is produced via site-specific conjugation of PE to Human SIRP alpha, Fc Tag under optimal conditions with a proprietary technology. Human SIRP alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).
Predicted N-terminus: Glu 31
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.7 kDa.
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
应用说明（Application）
Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
活性（Bioactivity）-FACS
Flow Cytometry assay shows that PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) can bind to Jurkat cells overexpressing human CD47. 1 μL stock solution per million cells (QC tested).
Protocol
FACS analysis shows that the binding of PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) to Jurkat cells overexpressing CD47 was inhibited by increasing concentration of neutralizing anti-human CD47 antibody. The IC50 is 0.37 μg/mL (QC tested).
Protocol
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