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分子别名（Synonym）
VEGFC,Flt4-L,VRP
表达区间及表达系统（Source）
Human VEGF-C Protein, His Tag (VEC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 103 - Arg 227 (Accession # AAH35212.1).
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 31 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
Unconjugated
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Human VEGF-C Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) with a linear range of 1-16 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) capture on NTA-Series S sensor chip can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with an affinity constant of 0.130 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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